Isolation and characterization of a plasmid from Pseudomonas fluorescens PF 13525

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Laurentian University , Sudbury, Ont
Statementby Denise Caughill.
The Physical Object
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Open LibraryOL22160675M

ISOLATION AND CHARACTERIZATION OF PSEUDOMONAS FLUORESCENS FROM RICE FIELDS T. Meera and P. Balabaskar Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, India ABSTRACT In the recent times, there has been a reversed interest in the search of plant growth promoting.

Description Isolation and characterization of a plasmid from Pseudomonas fluorescens PF 13525 EPUB

In this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets.

16S rRNA gene sequence of five isolates showed close homology with Pseudomonas putida, one with Pseudomonas graminis, and one with Pseudomonas by: Pseudomonas fluorescens (Pf1) and Bacillus subtilis (Bs4) isolates achieved maximum inhibition of radial growth of R.

bataticola by (%) and (%) respectively under in vitro study. Smit E, van Elsas J D, van Veen J A, de Vos W M. Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using phage R2f for donor counterselection.

Appl Environ Microbiol. ; – [Europe PMC free article] [Google Scholar]Cited by: Isolation and characterization of an aluminum‐sensitive mutant of Pseudomonas fluorescens Article in FEMS Microbiology Letters (2) - January with 24 Reads How we measure 'reads'.

isolation and partial characterization of plasmid dna from prooionibacterium abstract 30 introduction 31 materials and methods 33 results 42 discussion 58 acknowledgments 62 literature cited 63 manuscript ii. 67 characterization of propionibacterium plasmids abstract 69 introduction 70 materials and methods 73 results 83 discussion Pseudomonas fluorescens obtained from National Bureau of Agriculturally Important Insects-1(NBAII-Indian Council of Agricultural Research), Bangalore (PC1), and RomVijay Biotech, Puducherry (PC2), were used as controls.

Phenotypic level characterization of pseudomonads were carried out by subjecting the bacterial isolate. Isolation, Identification and Partial Characterization of Plasmid 10 representing %. From these stool cultures, 67(%) yielded a single enteric bacterial pathogen whereas 2(%) of the isolates revealed multiple kinds of pathogens.

Diarrhiagenic E. coli was the. Abstract. On the basis of rRNA homologies and the biology of pseudomonads, most species of RNA group I among the five RNA groups, such as Pseudomonas aeruginosa, P. putida, P.

Details Isolation and characterization of a plasmid from Pseudomonas fluorescens PF 13525 FB2

fluorescens, and P. syringae, belong to this organism (Palleroni et al., ).These Pseudomonas species play key roles in the environment, including the biodegradation of natural and man-made toxic chemicals and the.

Lurquin PF, Kado CI. Escherichia coli plasmid pBR insertion into plant protoplasts and into their nuclei. Mol Gen Genet. Jul 20; (2)– Manis JJ, Whitfield HJ. Physical characterization of a plasmid cointegrate containing an F'his gnd element and the Salmonella typhimurium LT2 cryptic plasmid.

J Bacteriol. The genus Pseudomonas represents a large group of medically and envi ronmentally important bacteria.

Interest in these bacteria is reflected in the extensive number of publications devoted to original research, re views, and books on this subject.

In this volume selected areas of Pseu domonas research are presented in depth by persons who have been active in their fields over many years.

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that the wide-host-range IncP-1 plasmid R (9) can also form such hybrids (11, 15, 18, 19). These R' plasmids have been constructed for Pseudomonas aeruginosa by using nosarecipients (15)orby using E.

coli as the recipient (11). The former method has the disadvantage that the resulting. A simple and rapid method was developed for the isolation of plasmid DNA from propionibacteria. Effective cellular lysis was achieved in all species when cells were treated with a high concentration of lysozyme.

Purification by acid phenol extraction or cesium chloride-density gradient centrifugation was required to remove contaminating chromosomal and open circular DNA and to obtain complete. Pseudomonas fluorescens ATCC T NR_ QVNA NZ_QVNA AJ Pseudomonas kilonensis DSM T NR_ AM AM NZ_LHVH Pseudomonas koreensis LMG T NR_ GU FN FN Pseudomonas mediterranea CFBP T NR_ AM AM.

Conjugation is an important gene transfer mechanism for bacteria in the soil and rhizosphere (7, 27, 28), and the genes responsible for mating-aggregate formation and DNA transfer are often carried by self-transmissible conjugative plasmids are known to be capable of recruiting chromosomal genes as well as mobilizing non-self-transmissible plasmids and hence can provide genetic.

The Pseudomonas strains were routinely grown at 28 °C on an in house medium, mediumcomposed of 10 g l −1 bacto tryptone, 5 g l −1 yeast extract, 5 g l −1 NaCl, 1 g l −1 glucose, g l −1 K 2 HPO 4 and g l −1 KH 2 PO 4.

Escherichia coli SM10 (λpir) was grown in at 37 °C. Gentamicin (Gm) was used at a concentration of 50 μg ml −1 (P.

fluorescens NCIMB ) or. Abstract. A total of different fluorescent Pseudomonas strains were isolated from several agricultural and industrial soils. The bacteria from the two different soil environments were compared for species and biotype variation, antibiotic and heavy metal resistance profiles, ability to degrade polyaromatic hydrocarbons, and plasmid incidence.

The antagonistic activity of three isolates of Pseudomonas fluorescens, 1–, 2–28 and 4–6, to control Botrytis cinerea (grey mold) under commercial cold storage conditions with two varieties of apple, ‘Ambrosia’ and ‘Spartan’, and possible mechanisms involved were investigated.

All three isolates of P. fluorescens significantly reduced the size of the lesion and the incidence. An alternative interpretation is that ATCC has been misidentified and is in fact P. fluorescens. An assessment by the Government of Canada of a strain of P. fluorescens (ATCC ) reached a similar conclusion to the current P.

putida assessment (Final Screening Assessment - Pseudomonas fluorescens ATCC ). None of the DSL strains. The ancestral strain of P. fluorescens SBW25 is a plasmid-free, non-pathogenic bacterium belonging to the rRNA group I of fluorescent Pseudomonas isolated from sugar beet phytosphere.

Escherichia coli DH5αλ pir [ 23 ] was used as a recipient strain for gene cloning and then a donor for conjugative transfer into Pseudomonas cells. Summary. Transfer of plasmid RP4 between introduced strains of Pseudomonas fluorescens was studied in 2 soils, Ede loamy sand and Guelph loam, in non-rhizosphere and rhizosphere soil using soil chambers and microcosm systems.

Short-term organism survival was generally at high levels (> 10 6 /g dry soil), in both soils, whereas long-term survival was poorer, particularly in the loamy sand. Hazard Assessment Characterization of Pseudomonas fluorescens Identification, Taxonomy and Strain History. Pseudomonas fluorescens is a Gram-negative, obligate aerobic, motile, rod-shaped bacterium.

It grows at neutral pH and has an optimal growth temperature of oC (Palleroni, ), with growth possible as low as 4oC. To pellet the plasmid DNA centrifuge at full speed for 15 minutes. After centrifugation, examine the tubes fo r a small white pellet of plasmid DNA.

Pour off the supernatants. Add µ l DNA Wash (70% isopropanol) to the pellets to wash away any excess salt. Une conclusion établie en vertu de la LCPE () sur la souche de Pseudomonas fluorescens ATCC ne présente pas un intérêt pour une évaluation, qu'elle n'empêche pas non plus, en fonction des critères de risque prévus dans le Système d'information sur les matières dangereuses utilisées au travail (SIMDUT), qui sont définis.

Plasmid dna isolation and profiling: Plasmid isolation was carried out using a commercial plasmid isolation kit (ZR Plasmid MiniprepTM- Classic with Catalog Number D4O15, D, and D4O54) as previously been described by Sambrook and Russel; ).

In brief, Zero point five millitre of the overnight culture was centrifuged. The. Degradative plasmids carry genes that confer on the host bacteria the ability to degrade recalcitrant organic compounds not commonly found in nature.

Many plasmid-encoded degradative gene clusters are also discrete regulons if they have regulators specialized for the regularion of the genes encoding degradative enzymes. The degradation pathway is composed of two segments when it is delimited. Spoilage induced by Pseudomonas strains is commonly found in a wide range of food products as a result of the ubiquitous presence of these strains and their ability to induce alteration through different mechanisms.

Particular attention has been recently paid on those P. fluorescens strains able to induce a blue discolouration on several food matrices (e.g. dairy or meat products).

Plasmids belonging to various incompatibility (Inc) groups were introduced into the efficiently root-colonizing strain Pseudomonas fluorescens WCS, and their stabilities in complex and minimal media and in the rhizospheres of tomato, wheat, and potato plants grown under gnotobiotic conditions without selection pressure were tested.

The IncP plasmid was found to be highly unstable under all. ISOLATION OF PLASMID DNA: ISOLATION OF PLASMID DNA Many methods have been developed to isolate plasmid DNA from the bacteria.

These, methods invariably involve three steps: Growth of the bacterial culture: Plasmids are always purified from cultures grown in liquid media containing appropriate antibiotics that have been inoculated with a single bacterial colony picked from an agar plate.

A set of self-transmissible plasmids with IncQ plasmid-mobilizing capacity was isolated by triparental exogenous isolation from the wheat rhizosphere with an Escherichia coli IncQ plasmid host and a Ralstonia eutropha recipient.

Three plasmids of 38 to 45 kb, denoted pIPO1, pIPO2, and pIPO3, were selected for further study. No selectable traits (antibiotic or heavy-metal resistance) were. Pseudomonas fluorescens is a common Gram-negative, rod-shaped bacterium. It belongs to the Pseudomonas genus; 16S rRNA analysis has placed P.

fluorescens in the P. fluorescens group within the genus, to which it lends its name.We report here the identification and characterization of mrdH, a novel chromosomal metal resistance determinant, located in the genomic island 55 of Pseudomonas putida KT It encodes for MrdH, a predicted protein of ∼40 kDa with a chimeric domain organization derived from the RcnA and RND (for resistance-nodulation-cell division) metal efflux proteins.Plasmid Isolation In order to obtain purified plasmid DNA for such procedures as cloning, PCR and transfection, plasmid isolation has to be performed.

The process involves using a number of techniques to obtain the plasmid DNA from host cells in order to use it in molecular biology.